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US 6,551,825 B1Granted
PiggyBac transposon-based genetic transformation system for insects
AssigneesThe United States of America as represented by the Secretary of Agriculture, University of Florida
InventorsPaul D Shirk, Malcolm J. Fraser, Teresa A. Elick, Omaththage P. Perera
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Abstract
The present invention is directed to nucleic acid and amino acid sequences for transformation constructs containing piggyBac or tagalong transposable elements. These constructs allow for the precise excision and insertion of heterologous DNA into a host cell.
Claims10
A transformed insect cell comprising
- (a) a first DNA comprising a non-transposon heterologous DNA sequence inserted between a pair of inverted repeats of a piggyBac transposon;
- and
- (b) a second DNA encoding a transposase active on a pair of inverted repeats, which second DNA is incapable of transposition caused by the transposase.
A transformed insect cell comprising
- (a) a non-transposon heterologous DNA sequence operably linked to an inducible promoter and inserted between a pair of inverted repeats of a piggyBac transposon;
- and
- (b) a second DNA encoding a transposase active on a pair of inverted repeats.
Dependencies are inferred from the claim text.