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US 6,551,814 B1Granted
Methods for bioremediation by degrading toluene
AssigneeOhio University
InventorPeter W. Coschigano
Life of this patent
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Abstract
Compositions and methods for the degradation of compounds contained in a liquid or solid waste stream are provided. Genes encoding toluene-degrading enzymes are also provided. The enzymes have homology to the E. coli pyruvate formate lyase and pyruvate formate lyase activator.
Claims8
A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of:
- a) providing
- i) a liquid or solid waste source comprising toluene,
- ii) a reaction containing means,
- iii) a functional, cell-free pyruvate formate lyase obtained from a toluene-degrading bacterium and iv) a functional, cell-free pyruvate formate lyase activating enzyme of a toluene-degrading bacterium;
- and
- b) reacting said functional, cell-free pyruvate fornate lyase of a toluene-degrading bacterium and said functional, cell-free pyruvate formate lyase activating enzyme of a toluene-degrading bacterium with said liquid or solid waste source in said reaction containing means under conditions such that toluene is degraded.
3 more elements
A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of:
- a) providing i) a liquid or solid waste source comprising toluene, and ii) a functional, cell-free pyruvate formate lyase tutD homologue obtained from a toluene-degrading bacterium selected from the group consisting of Thauera aromatics, Xanthomonas maltophilia, Geobacter metallireducens, and Azoarcus tolulyticus;
- b) reacting said functional, cell-free pyruvate formate lyase homologue with said liquid or solid waste source under conditions such that toluene is degraded.
A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of:
- a) providing
- i) a liquid or solid waste source comprising toluene,
- ii) a reaction containing means,
- iii) a functional, cell-free pyruvate fornate lyase having the amino acid sequence of SEQ ID NO:
- 16 and iv) a functional, cell-free pyruvate formate lyase activating enzyme having the amino acid sequence of SEQ ID NO:
- 18;
- and
- b) reacting said functional, cell-free pyruvate formate lyase and said functional, cell-free pyruvate formate lyase activating enzyme with said liquid or solid waste source in said reaction containing means under conditions such that toluene is degraded.
5 more elements
Dependencies are inferred from the claim text.