DSP-10 dual-specificity phosphatase
AssigneeCEPTYR, Inc.
InventorsRalf M Luche, Bo Wei
Life of this patent
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Abstract
Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-10, and polypeptide variants thereof that stimulate dephosphorylation of DSP-10 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents that inhibit DSP-10 activity. The polypeptides and agents may be used to modulate cell proliferation, differentiation and survival.
Claims10
An isolated polynucleotitie that encodes a polypeptide having a dual specificity phosphatase-10 (DSP-10) sequence as set forth in SEQ ID NO:2, or a variant thereof that differs in one or more amino acid deletions, additions, insertions or substitutions at no more than 25% of the residues in SEQ ID NO:2, such that the polypeptide retains the ability to dephosphorylate an activated MAP-kinase.
An isolated polynucleotidc, comprising a dual specificity phosphatase-10 (DSP-10) sequence as set forth in SEQ ID NO:1 that encodes a polypeptide sequence of DSP-10 as set forth in SEQ ID NO:2, or a variant thereof that differs in one or more amino acid deletions, additions, insertions, or substitutions at no more than 25% of the residues in SEQ ID NO:2, such that the polypeptide retains the ability to dephosphorylate an activated MAP-kinase.
An isolated polynucleotide that detectably hybridizes to a polynucleotide having a sequence that is complementary to a polynucleotide variant of SEQ ID NO:1, said variant comprising a polynucleotide encoding a dual specificity phosphatase-10 (DSP-10) polypeptide which comprises aspartic acid at position 377 of SEQ ID NO:2 and LLIHCQAGVSRSATTV (SEQ ID NO:3) at positions 404 through 419 of SEQ ID NO:2 under conditions that include a wash in 0.1×SSC and 0.1% SDS at 50° C. for 15 minutes, wherein said DSP-10 polypeptide retains the ability to dephosphorylate an activated MAP-kidnase.
An isolated polynucleotide comprising a nucleotide sequence that exhibits at least 70% nucleotide sequence identity to a nucleic acid sequence that encodes a dual specificity phosphatase-10 (DSP-10) polypeptide having an amino acid sequence as set forth in SEQ ID NO:2, said isolated polynucleotide encoding a dual specificity phosphatase-10 (DSP-10) polypeptide which comprises aspartic acid at position 377 of SEQ ID No:2 and LLIHCQAGVSRSATTV (SEQ ID NO:3) at positions 404 through 419 of SEQ ID NO:2, wherein said DSP-10 polypeptide retains the ability to dephosphorylate an activated MAP-kinase.
An expression vector comprising a polynucleotide according to any one of claims, 1, 2 or 4.
Dependencies are inferred from the claim text.