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US 6,551,798 B2Granted
Method for using a yeast alpha-amylase promoter
AssigneeBattelle Memorial Institute
InventorsJohnway Gao, Rodney S. Skeen, Brian S. Hooker, Daniel B. Anderson
Life of this patent
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Abstract
The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
Claims8
A method of expressing a gene product comprising:
- providing a host cell;
- introducing a DNA construct into the host cell, the construct comprising a nucleotide coding sequence that encodes a gene product operably linked to a promoter comprising at least nucleotides 1393 through 1961 of SEQ ID NO.:1;
- and
- expressing the gene product within the host cell.
1 more element
A host cell comprising a promoter operably linked to a coding sequence, the promoter comprising at least nucleotides 1393 through 1961 of SEQ ID NO.:6.
Dependencies are inferred from the claim text.