Stable biotinylated biomolecule composition
AssigneeE. R. Squibb & Sons, Inc.
InventorsSteven James Burton, James C. Pearson, Peter A. D. Edwardson, Alan Menzies
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Abstract
Stable compositions containing biotinylated molecules, such as enzymes, are provided. The compositions include a biotinylated biomolecule, a biomolecule protectant, a buffer, a bulking agent selected from one or more water soluble, nonionic polymers and preferably a terminal sterilization protectant. The compositions can be utilized either as aqueous solutions or preferably in dried form, e.g., as a lyophilized, powder cake. They have applicability in any case where avidin/biotin technology is used and are particularly important as compositions containing a thrombin-like enzyme, e.g., for preparation of a fibrin monomer and fibrin monomer-based fibrin sealants.
Claims25
A stable composition containing a biotinylated biomolecule comprising:
- said biotinylated biomolecule;
- a biomolecule protectant selected from the group consisting of an amino acid, trehalose, glycerol and ammonium sulphate;
- buffer means to maintain pH of said stable composition at a predetermined value;
- and one or more water soluble bulking agents;
- and further wherein said amino acid is a single zwitterion selected from the group consisting of glycine, alanine and valine.
A stable composition containing a biotinylated biomolecule comprising:
- said biotinylated biomolecule;
- a biomolecule protectant selected from the group consisting of an amino acid, trehalose, glycerol and ammonium sulphate;
- buffer means to maintain pH of said stable composition at a predetermined value;
- one or more water soluble bulking agents;
- and
- a terminal sterilization protectant selected from the group consisting of antioxidants, free radical scavengers and reducing agents;
- further wherein said antioxidants are selected from the group consisting of glutathione, α-tocopherol, N,N-dimethyl-p-phenylenediamine and sodium ascorbate.
A stable composition containing a biotinylated biomolecule comprising:
- said biotinylated biomolecule;
- a biomolecule protectant selected from the group consisting of an amino acid, trehalose, glycerol and ammonium sulphate;
- buffer means to maintain pH of said stable composition at a predetermined value;
- one or more water soluble bulking agents;
- wherein said composition is an aqueous solution and wherein said biotinylated biomolecule is present in an amount from about 50 to about 200 activity units per milliliter of said solution.
Dependencies are inferred from the claim text.