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US 6,551,790 B2Granted
Process for glucuronidation screening
AssigneePanVera Corporation
InventorsOlga Trubetskoy, Robert G. Lowery
Life of this patent
Estimating the term…
Abstract
A fluorescence polarization process used to identify activity of conjugative enzymes involved in xenobiotic transformations, such as glucuronosyltransferases is provided.
Claims8
A method of quantifying the amount of uridine diphosphate (UDP) in a sample comprising the steps of:
- a. incubating said sample with an enzyme capable of cleaving a phosphate group from said UDP under conditions that would allow said enzyme to be active;
- b. quenching said phosphate cleavage reaction;
- c. adding to said sample molybdenum/malachite green and incubating for a period of time sufficient for color development;
- and
- d. spectrophotometrically analyzing said sample.
2 more elements
A method of determining if a compound is a substrate for an UDP-glucoronosyltransferase comprising the steps of:
- a. providing a solution comprising a UDP-glucoronosyltransferase, uridine 5′-diphosphoglucuronic acid and an enzyme capable of removing a phosphate group from UDP, wherein said solution provides conditions that allow said UDP-glucoronosyltransferase and said enzyme to be active;
- b. adding said compound to said solution;
- c. quenching said phosphate cleavage reaction;
- d. adding to said sample molybdenum/malachite green and incubating for a period of time sufficient for color development;
- and
- e. spectrophotometrically analyzing said sample.
3 more elements
Dependencies are inferred from the claim text.