Motor proteins and methods for their use
AssigneeCytokinetics, Inc.
InventorsChristophe Beraud, Richard Freedman
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Abstract
The invention provides isolated nucleic acid and amino acid sequences of HsKif7, antibodies to HsKif7, methods of screening for HsKif7 modulators using biologically active HsKif7, and kits for screening for HsKif7 modulators.
Claims26
A method for screening for compounds that inhibit an activity of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises a sequence that has greater than 90% amino acid identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, the method comprising the steps of:
- contacting the target protein with a candidate agent at a first concentration and determining the target protein's ATPase activity;
- and
- contacting the target protein with a candidate agent at a second concentration and determining the target protein's ATPase activity;
- wherein a decrease in the level of ATPase activity of the target protein with an increase in concentration of the candidate agent indicates that the candidate agent inhibits the activity of the target protein.
A method for screening for compounds that activate an activity of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises a sequence that has greater than 90% amino acid identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, the method comprising the steps of:
- contacting the target protein with a candidate agent at a first concentration and determining the target protein's ATPase activity;
- and
- contacting the target protein with a candidate agent at a second concentration and determining the target protein's ATPase activity;
- wherein an increase in the level of ATPase activity of the target protein with an increase in concentration of the candidate agent indicates that the candidate agent activates the target protein.
Dependencies are inferred from the claim text.