Oligonucleotide specific of the Escherichia coli species and method for detecting and displaying bacteria of this species
AssigneeInstitut Pasteur
InventorsPatrick Grimont, Béatrice Regnault, Monique Collin
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Abstract
The invention concerns an oligonucleotide capable of being specifically hybridized with the ribosomal RNA (RNAr) or with the corresponding gene (ADNr) of the Escherichia coli genomic species (including all the Shigella genomic species except for serotype 13 S. boydii/Escherichia fergusonii genomic species. The invention also concerns a method for detecting and displaying the bacteria of said species.
Claims5
A procedure for detecting and visualizing bacteria of the genomic species Escherichia coli, wherein said genomic species comprises strains identified as Escherichia coli, Escherichia fergusonii, and all of the Shigella, with the exception of S. boydii serotype 13, comprising
- hybridizing ribosomal RNA of the bacteria of said genomic species with an oligonucleotide comprising SEQ ID NO:2;
- wherein hybridization between said ribosomal RNA and said oligonucleotide is indicative of the detection and visualization of said bacteria.
An oligonucleotide, which consists essentially of SEQ ID NO:2.
Dependencies are inferred from the claim text.