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US 6,551,772 B1Granted
Model for viral infection and immune response
AssigneeAntiCancer, Inc.
InventorsRobert M. Hoffman, Leonid B. Margolis
Life of this patent
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Abstract
An in vitro model system for viral infection and for immune response is comprised of a tissue block from adult tonsil or lymph node supported on a matrix which is flexible and porous and wherein the supported tissue block is cultured in a medium whose surface is congruent with the tissue block/matrix interface. The histoculture system can be used to screen for antiviral drugs, to monitor the course of viral diseases, and to monitor an immune response to antigen stimulation.
Claims4
A method to monitor the immune response to a recall antigen in vitro, which method comprises
- contacting a histoculture system with a recall antigen;
- and
- detecting antibody specific to said antigen, T cells and/or cytokines in response to said antigen as compared to a control histoculture which has not been provided said antigen, whereby the immune response is monitored by detecting the antibody, T cells, and/or cytokines;
- wherein said histoculture system comprises a flexible macromolecular, porous matrix, and supported thereon, an integral macroscopic section of animal lymph node tissue or tonsil tissue, said matrix immersed in a suitable culture medium wherein the surface of the medium is approximately congruent with the interface between the tissue and the matrix.
1 more element
Dependencies are inferred from the claim text.