Hyaluronidase preparation for ophthalmic administration and enzymatic methods for accelerating clearance of hemorrhagic blood from the vireous body of the eye

A method for inducing liquefaction of a vitreous humor to treat a disorder of a mammalian eye not as an adjunct to vitrectomy, said method comprising:

• contacting with the vitreous humor of the mammalian eye an amount of a solution which contains hyaluronidase to provide a dose of at least 1 International Unit of hyaluronidase, said solution being free of thimerosal, essentially devoid of hyaluronic acid-lysing material having a molecular weight greater than 100,000 as determined by 10% SDS PAGE electrophoresis, essentially devoid of caseinolytic material having a molecular weight greater than 45,000 as determined by 15% SDS PAGE electrophoresis, and essentially devoid of gelatinolytic material having a molecular weight between 60,000 and 100,000 as determined by 10% SDS PAGE electrophoresis, and wherein said method is carried out for the purpose of accelerating the clearance of hemorrhagic blood from the vitreous humor of the mammalian eye and wherein said contacting step is practiced in the absence of vitrectomy.