Electrochemical assays
AssigneeInverness Medical Switzerland GmbH
InventorsRobert Andrew Porter, Robert Andrew Badley
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Abstract
Disclosed is a component for a device for detecting the presence of an analyte of interest in a sample, the component comprising an electrically conducting solid support having immobilized thereon a chemical moiety, said chemical moiety comprising an electroactive portion with an electrochemical property capable of being directly modulated in a detectable manner by the binding thereto of a binding partner having a specific binding activity for the electroactive portion, together with apparatus comprising the component, and a method of detecting the presence of an analyte of interest.
Claims60
A detection component for use in a device for detection of an analyte in a sample, comprising:
- (a) an electrically conducting solid support;
- (b) a chemical moiety immobilized on the solid support, said chemical moiety comprising an electroactive portion;
- and
- (c) a binding entity reversibly immobilized on a solid surface within the component, said binding entity comprising:
- a first binding partner having specific binding activity for the chemical moiety, wherein binding of the first binding partner to the chemical moiety directly modulates an electrochemical property of the electroactive portion in a detectable manner;
- and
- a second binding partner, different from the first binding partner, selected such that the binding entity is released from the solid surface in the presence of analyte in a liquid medium.
An assay device for detection of an analyte of interest comprising:
- (a) a detection component comprising:
- (i) an electrically conducting solid support;
- (ii) a chemical moiety immobilized on the solid support, said chemical moiety comprising an electroactive portion;
- and
- (iii) a binding entity reversibly immobilized on a solid surface within the component, said binding entity comprising:
- a first binding partner having specific binding activity for the chemical moiety, wherein binding of the binding partner to the chemical moiety directly modulates an electrochemical property of the electroactive portion in a detectable manner;
- and
- a second binding partner, different from the first binding partner, selected such that the binding entity is released from the solid support in the presence of analyte in a liquid medium;
- and
- (b) one of more additional components selected from among sample receiving means for accepting a sample and bringing it into contact with the detection component, detection means for detecting the modulation in the electrochemical property of the electroactive portion of the chemical moiety, and data display means for displaying an assay result.
A method for detecting an analyte comprising the steps of:
- (a) placing a liquid sample to be assayed for the presence of the analyte in contact with
- (i) an electrically conducting solid support having immobilized thereon a chemical moiety, said chemical moiety comprising an electroactive portion;
- and
- (ii) a binding entity, said binding entity comprising:
- a first binding partner having specific binding activity for the chemical moiety, wherein binding of the binding partner to the chemical moiety directly modulates an electrochemical property of the electroactive portion in a detectable manner, and
- a second binding partner, different from the first binding partner, said second binding partner being an analyte-specific binding agent or an analyte analog;
- and
- (b) detecting modulation of the electrochemical property of the electroactive portion, wherein such modulation occurs only in the presence of analyte.
Dependencies are inferred from the claim text.