Methods for creating transgenic animals
AssigneeWisconsin Alumni Research Foundation
InventorsRobert D. Bremel, Anthony W.S. Chan, Jane C. Burns
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Abstract
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
Claims11
A method for the production of a transgenic bovine embryo comprising:
- a) providing:
- i) a bovine unfertilized egg;
- ii) an aqueous solution containing infectious retrovirus which includes a polynucleotide encoding a protein of interest;
- b) introducing said solution containing infectious retrovirus into said oocyte under conditions which permit the infection of sad oocyte, thereby giving an infected oocyte;
- and
- c) contacting said infected oocyte with sperm under conditions which permit the fertilization of said infected oocyte to produce an embryo, said embryo comprising a copy of the polynucleotide integrated therein.
Dependencies are inferred from the claim text.