7B2 knockout transgenic animals as models of endocrine disease
AssigneesPresident and Fellows of Harvard College, Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorsChristoph H. Westphal, Iris Lindberg, Philip Leder
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Abstract
In general, the invention features methods and uses for transposon-mediated gene targeting which greatly enhance the insertion and detection of desired genes in genomic exons by homologous recombination. The invention also features transgenic non-human mammals, and eukaryotic cells, wherein a gene encoding 7B2 protein is modified, as well as nucleic acid vectors capable of undergoing homologous recombination with an endogenous 7B2 gene in a cell. The invention also features transgenic non-human mammals as models of endocrine disorders, as well as methods for diagnosing and treating patients with endocrine disorders.
Claims10
A transgenic postnatal mouse whose genome is disrupted by recombination at each 7B2 gene locus so as to produce a phenotype, relative to a wild-type phenotype, comprising absence of PC2 activity in the brain of said transgenic mouse.
A postnatal transgenic mouse, homozygous for a loss of function mutation in each 7B2 gene, which loss of function mutation is created by recombination with heterologous nucleotide sequences, wherein said transgenic mouse has a phenotype including at least one of hypoglycemia, hyperproinsulinemia or hypercortisolism.
A transgenic mouse, whose genome comprises a homozygous disruption of both 7B2 loci by insertion of a transgene including a selectable marker sequence, wherein said disruption results in said transgenic mouse having a phenotype including at least one of hypoglycemia, hyperproinsulinemia or hypercortisolism.
A postnatal transgenic knock-out mouse whose genome is disrupted by recombination at each 7B2 gene locus so as to produce a phenotype, relative to a wild-type phenotype, including at least one of hypoglycemia, hyperproinsulinemia, hypercortisolism, increased plasma ACTH or increased serum corticosterone.
Dependencies are inferred from the claim text.