Production of heterologous protein in milk of transgenic non-human mammals comprising a construct with an intron
AssigneePPL (Holdings) Limited
InventorsAlan Langskill Archibald, Anthony John Clark
Life of this patent
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Abstract
Proteinaceous products can be produced by transgenic animals having genetic constructs integrated into their genome. The construct comprises a 5′-flanking sequence from a mammalian milk protein gene (such as beta-lactoglobulin) and DNA coding for a heterologous protein other than the milk protein (for example a serin protease such as alpha1-antitrypsin or a blood factor such as Factor VIII or IX). The protein-coding DNA comprises at least one, but not all, of the introns naturally occurring in a gene coding for the heterologous protein. The 5′-flanking sequence is sufficient to drive expression of the heterologous protein.
Claims10
A method for producing a heterologous protein, the method comprising expressing said heterologous protein in the milk of a non-human transgenic placental mammal and harvesting the heterologous protein, wherein said non-human transgenic placental mammal has integrated into its genome a genetic construct comprising
- (a) a 5′-flanking sequence from a mammalian milk protein gene and
- (b) DNA coding for said heterologous protein other than said milk protein, wherein said DNA comprises at least one whole, but not all, of the introns naturally occurring in the gene coding for said heterologous protein and
- wherein said 5′-flanking sequence controls expression of said heterologous protein, said genetic construct being integrated into said non-human transgenic placental mammal's genome in such a way that said DNA is expressed in a mammary gland of said mammal at levels greater than those achieved by expression of a corresponding intron-less construct.
Dependencies are inferred from the claim text.