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US 6,548,657 B1Granted
Method for screening nucleic acid catalysts
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsAlex Burgin, Leonid Beigelman, Laurent Bellon
Life of this patent
Estimating the term…
Abstract
Nucleic acid catalysts, method of screening for variants of nucleic acid catalysts, synthesis of ribozyme libraries and discovery of gene sequences are described.
Claims24
An isolated and purified nucleic acid molecule with an endonuclease activity having formula I:
- wherein, N is independently a nucleotide or a non-nucleotide linker, which may be same or different;
- M and Q are independently oligonucleotides of sufficient length to stably interact with a target nucleic acid molecule;
- o and n are integers greater than or equal to 1, wherein if (N)o and (N)n are nucleotides, (N)o and (N)n are optionally able to interact by hydrogen bond interaction;
- L is a linker which may be present or absent, but when present, is a nucleotide and/or a non-nucleotide linker, which may be a single-stranded and/or a double-stranded region;
- represents a chemical linkage;
- and A, C, U and G represent adenosine, cytidine, uridine and guanosine nucleotides, respectively.
3 more elements
A nucleic acid molecule with catalytic activity having the formula II:
- wherein, N is independently a nucleotide or a non-nucleotide linker, which may be same or different;
- M and Q are independently oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- o and n are integers greater than or equal to 1, wherein if (N)o and (N)n are nucleotides, (N)o and (N)n are optionally able to interact by hydrogen bond interaction;
- L is a linker which may be present or absent, but when present, is a nucleotide and/or a non-nucleotide linker, which may be a single-stranded and/or double-stranded region;
- Z3 is 2′-methylthiomethyl uridine;
- Z4 is 2′-C-allyl uridine;
- Z7 is 6-methyl uridine;
- _ represents a chemical linkage;
- and A, and G represent adenosine and guanosine nucleotides, respectively.
6 more elements
A nucleic acid molecule with catalytic activity having the formula III:
- wherein, N is independently a nucleotide or a non-nucleotide linker, which may be same or different;
- M and Q are independently oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- o and n are integers greater than or equal to 1, wherein if (N)o and (N)n are nucleotides, (N)o and (N)n are optionally able to interact by hydrogen bond interaction;
- L is a linker which may be present or absent, but when present, is a nucleotide and/or a non-nucleotide linker, which may be a single-stranded and/or double-stranded region;
- Z3 is 2′-methylthiomethyl uridine;
- Z4 is 2′-methylthiomethyl cytidine;
- Z7 is 6-methyl uridine;
- represents a chemical linkage;
- and A, and G represent adenosine and guanosine nucleotides, respectively.
6 more elements
A nucleic acid molecule with catalytic activity having the formula IV:
- wherein, N is independently a nucleotide or a non-nucleotide linker, which may be same or different;
- M and Q are independently oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- o and n are integers greater than or equal to 1, wherein if (N)o and (N)n are nucleotides, (N)o and (N)n are optionally able to interact by hydrogen bond interaction;
- L is a linker which may be present or absent, but when present, is a nucleotide and/or a non-nucleotide linker, which may be a single-stranded and/or double-stranded region;
- Z3 is 2′-methylthiomethyl uridine;
- Z4 is 2′-methylthiomethyl cytidine;
- Z7 is 2′-C-allyl uridine;
- represents a chemical linkage;
- and A, and G represent adenosine and guanosine nucleotides, respectively.
6 more elements
A nucleic acid molecule with catalytic activity having the formula V:
- wherein, N is independently a nucleotide or a non-nucleotide linker, which may be same or different;
- M and Q are independently oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- o and n are integers greater than or equal to 1, wherein if (N)o and (N)n are nucleotides, (N)o and (N)n are optionally able to interact by hydrogen bond interaction;
- L is a linker which may be present or absent, but when present, is a nucleotide and/or a non-nucleotide linker, which may be a single-stranded and/or double-stranded region;
- Z3 is 2′-methylthiomethyl uridine;
- Z4 is 2′-methylthiomethyl cytidine;
- Z7 is pyridine-4-one;
- and_ represents a chemical linkage;
- and A, and G represent adenosine and guanosine nucleotides, respectively.
6 more elements
Dependencies are inferred from the claim text.