Interleukin-4 receptors
AssigneeImmunex Corporation
InventorsBruce Mosley, David J. Cosman, Linda Park, M. Patricia Beckmann, Carl J. March, Rejean Idzerda
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Abstract
Mammalian Interleukin-4 receptor proteins, DNAs and expression vectors encoding mammalian IL-4 receptors, and processes for producing mammalian IL-4 receptors as products of cell culture, are disclosed. A method for suppressing an IL-4-dependent immune or inflammatory response in a mammal, including a human, by administering an effective amount of soluble IL-4 receptor (sIL-4R) and a suitable diluent or carrier.
Claims46
An isolated DNA which encodes a soluble human IL-4R that binds ILA, fused to a second polypeptide that is not derived from the human IL-4R of FIGS. 4A-4C;
- wherein the soluble human IL-4R comprises amino acids 1-207 of FIG. 4A.
An isolated DNA which encodes a soluble human IL-4R that binds IL-4, fused to a second polypeptide that is not derived from the human IL-4R of FIGS. 4A-4C;
- wherein the soluble human IL-4R comprises amino acids 1-197 of FIG. 4A.
An isolated DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises an IL-4 Receptor (IL-4R) polypeptide and a second polypeptide, wherein the IL-4R polypeptide is selected from the group consisting of:
- a) a polypeptide comprising amino acids −25 to 800 of FIGS. 4A-4C;
- and
- b) a fragment of the polypeptide of (a), wherein said fragment binds IL-4.
An isolated DNA comprising a nucleotide sequence encoding a fusion protein that comprises a soluble fragment of the human IL-4R protein of FIGS. 4A-4C, wherein said IL-4R fragment binds IL-4, and a second polypeptide that is neither the full length IL-4R of FIGS. 4A-4C nor a fragment of the IL-4R of FIGS. 4A-4C.
An isolated DNA comprising a nucleotide sequence that encodes a soluble fusion protein comprising:
- an IL-4R polypeptide, wherein the IL-4R polypeptide is a soluble form of the human IL-4R protein of FIGS. 4A-4C, with the proviso that the IL-4R polypeptide contains an amino acid residue selected from the group consisting of isoleucine and valine at the position corresponding to position 50 in the sequence presented in FIG. 4A,
- and a second polypeptide other than a full length or soluble form of the protein of FIGS. 4A-4C,
- wherein the fusion protein binds IL-4.
An isolated DNA comprising a nucleotide sequence encoding a soluble fusion protein that comprises:
- (a) a soluble IL-4R polypeptide that binds IL-4, comprising an amino acid sequence that differs by one amino acid deletion, insertion or substitution from the sequence of amino acids 1 to x of FIG. 4A, wherein x represents an integer from 197 through 207, and (b) a second polypeptide distinct from an IL-4R polypeptide as defined in (a).
An isolated DNA comprising a nucleotide sequence encoding a soluble fusion protein that comprises a soluble IL-4R polypeptide that binds IL-4, wherein the IL-4R comprises an amino acid sequence selected from the group consisting of:
- a) an amino acid sequence comprising residues 1-197 of FIG. 4A;
- and
- b) an amino acid sequence that includes a substitution in the sequence of (a);
- said fusion protein additionally comprising a second polypeptide that does not comprise an amino acid sequence of (a) or (b).
An isolated DNA comprising a nucleotide sequence encoding a soluble fusion protein that comprises:
- (a) a soluble IL-4R polypeptide that binds IL-4, comprising an amino acid sequence that is at least 80% identical to the sequence of amino acids 1-207 of FIG. 4A, and (b) a second polypeptide distinct from an IL-4R polypeptide as defined in (a).
Dependencies are inferred from the claim text.