Synthetic genes for plant gums
AssigneeOhio University
InventorMarcia J. Kieliszewski
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Abstract
A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
Claims17
A purified plant gum polypeptide consisting essentially of repeating units which consist of at least a portion of an amino acid sequence selected from the group consisting of Ser-Hyp-Hyp-Hyp-Hyp-Leu-Ser-Hyp-Ser-Hyp-Thr-Hyp-Thr-Hyp-Hyp-Hyp-Gly-Pro-His (SEQ ID NO:113) and Ser-Hyp-Hyp-Hyp-Thr-Leu-Ser-Hyp-Ser-Hyp-Thr-Hyp-Thr-Hyp-Hyp-Hyp-Gly-Pro-His (SEQ ID NO:114), or variants comprising a single amino acid substitution of said amino acid sequences, wherein said portion is between thirteen and nineteen contiguou amino acids.
A purified plant gum polypeptide consisting essentially of repeating units which consist of a first region and a second region, said first region comprising and amino acid sequence selected from the group consisting of Ser-Hyp-Hyp-Hyp-Hyp-Leu (SEQ ID NO:117) and Ser-Hyp-Hyp-Hyp-Thr-Leu (SEQ ID NO:118), and said second region comprising an amino acid sequence Ser-Hyp-Ser-Hyp-Thr-Hyp-Thr-Hyp-Hyp-Hyp-Gly-Pro-His (SEQ ID NO:20), or variants comprising a single amino acid substitution of said amino acid sequences.
A purified plant gum polypeptide, consisting essentially of repeating units of at least a portion of an amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, and SEQ ID NO:28, wherein said portion is between thirteen and nineteen contiguous amino acids.
Dependencies are inferred from the claim text.