Pesticidal toxins
AssigneeMycogen Corporation
InventorsKenneth E. Narva, H. Ernest Schnepf, Mark Knuth, Michael R. Pollard, Guy A. Cardineau, George E. Schwab, Tracy Ellis Michaels
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Abstract
The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.
Claims47
An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of the nucleotide sequence represented by SEQ ID NO:10 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of a nucleotide sequence that codes for the amino acid sequence represented by SEQ ID NO:11 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
An isolated polynucleotide that encodes a Bacillus toxin active against a corn rootworm pest wherein said toxin comprises the amino acid sequence of SEQ ID NO:43.
An isolated polynucleotide comprising a nucleotide sequence that encodes an approximately 40-50 kDa toxin that is active against a corn rootworm pest wherein said toxin is from Bacillus thuringiensis isolate PS149B1 having accession number NRRL B-21553 and mutants thereof that retain pesticidal activity.
A transgenic cell that expresses an isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the fill complement of a nucleotide sequence that codes for the amino acid sequence represented by SEQ ID NO:11 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein, and wherein said cell is selected from the group consisting of a plant cell and a microbial cell.
A transgenic cell that expresses an isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of the nucleotide sequence represented by SEQ ID NO:10 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
A transgenic cell that expresses an isolated polynucleotide comprising a nucleotide sequence that encodes an approximately 40-50 kDa toxin that is active against a corn rootworm pest wherein said toxin is from Bacillus thuringiensis isolate PS149B1 having accession number NRRL B-21553 and mutants thereof that retain pesticidal activity.
A transgenic cell that expresses an isolated polynucleotide that encodes a Bacillus toxin active against a corn rootworm pest wherein said toxin comprises the amino acid sequence of SEQ ID NO:43.
Dependencies are inferred from the claim text.