Polynucleotides encoding Aspergillus Niger and Penicillium Chrysogenum acetamidases and methods of use as selectable markers
AssigneeDsm N.V.
InventorsBart W. Swinkels, Gerardus C. M. Selten, Janna G. Bakhuis, Roelof A. L. Bovenberg, Adrianus W. H. Vollebregt
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Abstract
The present invention discloses novel amdS genes from fungi previously not known to contain an amdS gene, such as Aspergillus niger and Penicllium chrysogenum. The novel amdS genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amdS genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.
Claims13
A purified and isolated nucleic acid molecule that comprises an acetamidase-encoding nucleotide sequence that is selected from the group consisting of SEQ ID NO:18 and SEQ ID NO:19.
A recombinant nucleic acid molecule that comprises an acetamidase-encoding nucleotide sequence that is selected from the group consisting of SEQ ID NO:18 and SEQ ID NO:19.
A recombinant nucleic acid molecule that comprises an acetamidase-encoding nucleotide sequence that is selected from the group consisting of SEQ ID NO:18 and SEQ ID NO:19, said encoding nucleotide sequence operably linked to a promoter.
Dependencies are inferred from the claim text.