D-enzyme compositions and methods of their use
AssigneeThe Scripps Research Institute
InventorsStephen Brian Henry Kent, Saskia Charlotte Florence Milton, Raymond Cecil deLisle Milton
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Abstract
D-enzyme compositions are described comprising an amino acid residue sequence that defines an polypeptide able to catalyze an enzymatic reaction. The D-enzyme has an amino acid residue sequence consisting of D-amino acids and glycine.
Claims8
A synthetic D-enzyme corresponding to a natural L-enzyme, wherein the synthetic D-enzyme has a D-amino acid residue sequence consisting of D-amino acids and glycine that defines a D-polypeptide able to catalyze a first enzymatic reaction corresponding to a second enzymatic reaction catalyzed by the natural enzyme,
- said D-amino acid residue sequence corresponding to an L-amino acid residue sequence defined by the natural L-enzyme,
- said D-polypeptide having a conformation that corresponds to a mirror image of an L-polypeptide defined by the natural L-enzyme.
A method for producing a chirally pure chemical comprising:
- a) reacting in an aqueous admixture a first stereoisomer with a synthetic D-enzyme that specifically converts said first stereoisomer into a chiral reaction product, said reacting occurring for a time period and under reaction conditions sufficient to form said reaction product, wherein said D-enzyme having an amino acid residue sequence that defines a polypeptide able to catalyze said reaction, said amino acid residue sequence consisting of D-amino acids and glycine, said polypeptide having a conformation that is a mirror image of a natural L-enzyme;
- and
- b) isolating said chiral reaction product from said admixture, thereby forming said chirally pure chemical.
A method for producing a chirally pure chemical comprising:
- a) reacting in an aqueous admixture a racemic mixture having at least a first and a second stereoisomer with a synthetic D-enzyme that specifically converts said first stereoisomer into a reaction product, said reacting occurring for a time period and under reaction conditions sufficient to convert substantially all of said first stereoisomer into said reaction product, wherein said D-enzyme having an amino acid residue sequence that defines a polypeptide able to catalyze said reaction, said amino acid residue sequence consisting of D-amino acids and glycine, said polypeptide having a conformation that corresponds to a mirror image of a natural L-enzyme;
- and
- b) isolating said second stereoisomer from said admixture, thereby forming said chirally pure chemical.
Dependencies are inferred from the claim text.