Alzheimer model for drug screening
AssigneeCase Western Reserve University
InventorsMark A. Smith, Andrew McShea
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Abstract
The present invention relates to methods and reagents for drug screening. Screening methods are disclosed employing neuronal cells that have been treated so as to exhibit characteristics associated with a re-entry into the cell cycle. One treatment approach comprises transfection of neuronal cells with an expression vector comprising one or more oncogenes.
Claims6
A screening method for testing compounds for their ability to inhibit a deleterious event associated with re-entry into the cell cycle comprising:
- a) providing, in any order:
- i) a reaction vessel;
- ii) a plurality of mammalian neuronal cells transfected with two oncogenes such that said transfected cells exhibit said deleterious event associated with reentry into the cell cycle, wherein said oncogenes are Myc and Ras;
- and
- iii) a test compound;
- b) combining said transfected cells and said test compound within said reaction vessel under conditions such that said compound is free to interact with said transfected cells;
- c) detecting said interaction under conditions such that the amount of inhibition of said deleterious event associated with re-entry into the cell cycle is measured;
- and
- d) determining the viability of said cells after contact with said test compound.
A screening method for testing compounds for their ability to inhibit the phosphorylation of the tau protein comprising:
- a) providing, in any order:
- i) a reaction vessel;
- ii) a plurality of mammalian neuronal cells transfected with two oncogenes such that said transfected cells exhibit phosphorylation of the tau protein, wherein said oncogenes are Myc and Ras;
- and
- iii) a test compound;
- b) combining said transfected cells and said test compound within said reaction vessel under conditions such that said compound is free to interact with said transfected cells;
- and
- c) detecting said interaction under conditions such that the amount of inhibition of said phosphorylation is measured.
Dependencies are inferred from the claim text.