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US 6,548,244 B2Granted
Process for typing HCV isolates
AssigneeInnogenetics N.V.
InventorsGeert Maertens, Lieven Stuyver, Rudi Rossau, Hugo Van Heuverswyn
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AssigneeInnogenetics N.V.
InventorsGeert Maertens, Lieven Stuyver, Rudi Rossau, Hugo Van Heuverswyn
Estimating the term…
The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.
A method for detecting the presence of an infection with an HCV virus in a biological sample on the basis of the presence of a genotype-specific sequence or an isolate-specific mutation present in any of SEQ ID NO 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 or 81.
A method for detecting the presence of an infection with an HCV virus in a biological sample on the basis of the presence of any of SEQ ID NO 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 or 81.
A method comprising the determination of the presence in a biological sample of at least one of the following variable nucleotide regions or positions of any HCV 5′UTR sequence by means of a sequencing reaction, wherein said method detects the nucleotide variation present between HCV strains in at least one of the following regions of HCV:
A method for detecting the presence of an infection with an HCV virus in a biological sample on the basis of the presence of a genotype-specific sequence or an isolate-specific mutation present in a nucleic acid encoding any of SEQ ID NOs 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92.
A method for detecting the presence of an infection with an HCV virus in a biological sample on the basis of the presence of a nucleotide sequence encoding any of SEQ ID NOs 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92.
A method for detecting the presence of an infection with an HCV virus in a biological sample on the basis of the presence of at least part of an amino acid sequence of any of SEQ ID NOs 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92.
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