Stabilized particles and methods of preparation and use thereof
AssigneeThe University of Melbourne
InventorsPaul Charles Mulvaney, Luis Manuel Liz-Marzan
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Abstract
A method of stabilizing particles with an insulating, semiconducting and/or metallic coating and stabilized particles prepared thereby are disclosed. In addition, particles stabilized by an insulating, semiconducting and/or metallic coating, wherein said coating is attached to said particles via a bifunctional ligand are provided, as is a method is provided for determining the presence of an analyte in a sample comprising incubating the sample with a ligand bound to a coated particle, capable of specifically binding to the analyte and capable of providing a detectable signal and detecting the presence of a ligand-coated particle:analyte complex as indicating the presence of the analyte in the sample.
Claims53
A method for preparing a coated particle, comprising:
- (i) admixing a source of the particle with a source of coating to provide a particle-coating admixture;
- (ii) adding to the particle-coating admixture a bifunctional ligand having structural formula (I):
- A—X—B
- wherein A is a first functional group that attaches to the particle, or to a coating formed on the particle, B is a second functional group that activates the surface of the core particle for nucleation of a coating layer and X is an optional linking group;
- and
- (iii) allowing the bifunctional ligand and coating to deposit on the particle.
A method for preparing a coated particle, comprising:
- (i) admixing a source of the particle with a bifunctional ligand having structural formula (I):
- A—X—B
- wherein A is a first functional group that attaches to the particle, or to a coating formed on the particle, B is a second functional group that activates the surface of the core particle for nucleation of a coating layer and X is an optional linking group, to provide a particle-ligand admixture;
- (ii) adding to particle-ligand admixture a source of coating;
- and
- (iii) allowing the bifunctional ligand and coating to deposit on the particle.
A particle stabilized by a coating, wherein said coating is attached to said particle via a bifunctional ligand having structural formula (I):
- A—X—B
- wherein A is a first functional group that attaches to the particle, or to a coating formed on the particle, B is a second functional group that activates the surface of the core particle for nucleation of a coating layer and X is an optional linking group.
A method for determining the presence of an analyte in a sample suspected of containing the analyte comprising:
- (a) providing a coated particle-ligand composition, wherein the ligand is bound to the coated particle and further wherein the coated particle-ligand composition is capable of specifically binding to the analyte and capable of providing a detectable signal;
- (b) incubating the sample with the coated particle-ligand composition to form a coated particle-ligand:analyte complex;
- and
- (c) detecting the presence of the coated particle-ligand:analyte complex as indicating of the presence of the analyte in the sample and wherein the coated particle comprises a coating attached to said particle via a bifunctional ligand having structural formula (I):
- A—X—B
- wherein A is a first functional group that attaches to the particle, or to a coating formed on the particle, B is a second functional group that activates the surface of the core particle for nucleation of a coating layer and X is an optional linking group.
Dependencies are inferred from the claim text.