Interleukin-15 receptors
AssigneeImmunex Corporation
InventorsDirk M. Anderson, Judith G. Giri
Life of this patent
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Abstract
There are disclosed Interleukin-15 Receptor (IL-15R) proteins, DNAs and expression vectors encoding IL-15R, and processes for producing IL-15R as products of recombinant cell cultures.
Claims28
An isolated IL-15 receptor (IL-15R) encoded by a DNA selected from the group consisting of:
- (a) a DNA comprising nucleotides 34 through 753 of SEQ ID NO:6;
- (b) DNAs that hybridize to the DNA sequence of (a) or its complementary strand under moderately stringent conditions (50° C., 2×SSC), and which encode polypeptides capable of binding IL-15;
- and
- (c) DNAs that, due to degeneracy of the genetic code, encode polypeptides encoded by any of the foregoing DNAs.
An isolated IL-15 receptor (IL-15R) polypeptide that is at least 80% identical to amino acids 12-251 of SEQ ID NO:7 or a fragment thereof;
- wherein the polypeptide and fragment thereof are capable of binding IL-15.
An isolated soluble IL-15 receptor (IL-15R) polypeptide that is at least 80% identical to amino acids 12-189 of SEQ ID NO:7, or a fragment thereof;
- wherein the polypeptide and fragment thereof are capable of binding IL-15.
An isolated soluble IL-15 receptor (IL-15R) polypeptide comprising amino acids 12-251 of SEQ ID NO:7 or a fragment thereof that is capable of binding IL-15.
An isolated polypeptide produced by a process comprising culturing a recombinant host cell under conditions promoting expression of the polypeptide and recovering the polypeptide, wherein the recombinant host cell comprises a DNA selected from the group consisting of:
- (a) a DNA encoding SEQ ID NO:15, wherein residue 182 is Thr or Asn;
- (b) a DNA comprising the sequence from ATCC 69690 that encodes murine IL-17 leader sequence fused to a sequence encoding mature IL-15R;
- (c) a DNA comprising nucleotides 83-784 of SEQ ID NO:10;
- (d) a DNA that hybridizes to a DNA of (a), (b) or (c), or its complementary strand, under moderately stringent conditions (50° C., 2×SSC), and encodes a polypeptide capable of binding IL-15;
- (e) a DNA that encodes a polypeptide capable of binding IL-15, wherein the polypeptide is at least 80% identical to a polypeptide encoded by the DNA of (a), (b), (c) or (d);
- and
- (f) a DNA that, due to degeneracy of the genetic code, encodes a polypeptide encoded by any of the foregoing DNAs.
An isolated IL-15 receptor (IL-15R) polypeptide comprising a sequence selected from the group consisting of:
- (a) amino acids 1-234 of SEQ ID NO:11, and fragments thereof that are capable of binding IL-15;
- (b) sequences that are at least 80% identical to (a), wherein the polypeptide is capable of binding IL-15;
- (c) sequences encoded by a DNA that hybridizes to nucleotides 83-784 of SEQ ID NO:10 and encodes a polypeptide capable of binding IL-15;
- (d) amino acids 1-225 of SEQ ID NO:14, and fragments thereof that are capable of binding IL-15;
- and
- (e) sequences that are at least 80% identical to (d), wherein the polypeptide is capable of binding L-15.
Dependencies are inferred from the claim text.