US 6,548,046 B1 · Site specific binding system, imaging compositions and methods

Abstract

A method for ligand-based binding of lipid encapsulated particles to molecular epitopes on a surface in vivo or in vitro comprises sequentially administering (a) a site-specific ligand activated with a biotin activating agent; (b) an avidin activating agent; and (c) lipid encapsulated particles activated with a biotin activating agent, whereby the ligand is conjugated to the particles through an avidin-biotin interaction and the resulting conjugate is bound to the molecular epitopes on such surface. The conjugate is effective for imaging by x-ray, ultrasound, magnetic resonance or positron emission tomography. Compositions for use in ultrasonic imaging of natural or synthetic surfaces and for enhancing the acoustic reflectivity thereof are also disclosed.

Claims12

Dependent claims

A method for enhanced ultrasound imaging of a target tissue in a subject in vivo which method comprises

detecting an ultrasound image of said target tissue wherein said target tissue is bound to a ligand specific for said target tissue and wherein said ligand is directly coupled to lipid-coated liquid perfluorocarbon particles contained in an emulsion,
which method further comprises, prior to said detecting, administering said emulsion to said subject.

Dependencies are inferred from the claim text.

Facts

Application

09/404,963Filed 24 Sep 1999 · continuation in part

Priority

8 Jun 1995

Granted

15 Apr 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

A61K 49/18
Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations: characterised by a special…
A61K 49/1806
Characterised by a special physical form, e.g. emulsions, microcapsules, liposomes: suspensions, emulsions, colloids, dispersions
A61K 51/1217
Characterised by a special physical form, e.g. emulsion, microcapsules, liposomes , characterized by a special physical form, e.g. emulsions,…:…
G01N 33/586
With a particulate label, e.g. coloured latex: liposomes, microcapsules or cells

Family

Application08/488,743Parent of this division · filed 8 Jun 1995

Application08/854,308Parent of this continuation · filed 12 May 1997

US 5,690,907 AParent of this division · filed 25 Nov 1997

Application09/189,118Parent of this continuation-in-part · filed 9 Nov 1998

Application09/189,118Parent of this continuation

Application08/854,308Parent of this division

US 6,548,046 B1This patent

From the US filing history. Foreign family members aren’t listed.

Site specific binding system, imaging compositions and methods

Life of this patent

Site specific binding system, imaging compositions and methods

Life of this patent

Abstract

Claims12