Biosensor and method for quantitating biochemical substrate using the same
AssigneeMatsushita Electric Industrial Co., Ltd.
InventorsShin Ikeda, Tomohiro Yamamoto, Toshihiko Yoshioka, Shiro Nankai, Shigeki Joko, Junko Iwata
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Abstract
A biosensor includes: an insulating substrate; an electrode system formed on the insulating substrate which has a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and an electron acceptor. The electron acceptor is ferricinium ion derived from ferrocene electrolyte.
Claims14
A biosensor comprising:
- an insulating substrate;
- an electrode system formed on the insulating substrate which comprises a working electrode and a counter electrode;
- and
- a solid phase reaction layer formed on the insulating substrate which contains a solid phase oxidoreductase and a solid phase ferrocene electrolyte as an electron acceptor, the solid phase reaction layer being formed over at least a portion of the working electrode or the counter electrode,
- wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion, and wherein the ferricinium ion is provided in the biosensor together with the oxidoreductase upon dissolution of the reaction layer.
A method for quantitating the concentration of a biochemical substrate in a sample liquid by using a biosensor comprising an insulating substrate, an electrode system formed on the insulating substrate which has a working electrode and a counter electrode and a solid phase reaction layer provided on the insulating substrate which contains a solid phase oxidoreductase and a solid phase ferrocene electrolyte as an electron acceptor, the solid phase reaction layer being formed over at least a portion of the working electrode or the counter electrode, comprising the steps of:
- adding the sample liquid to the reaction layer;
- and
- detecting a response current value by applying a voltage between the working electrode and counter electrode,
- wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion, and wherein the ferricinium ion is provided in the biosensor together with the oxidoreductase upon dissolution of the reaction layer.
A biosensor comprising:
- an insulating substrate;
- an electrode system formed on the insulating substrate which comprises a working electrode and a counter electrode;
- and
- a solid phase reaction layer formed on the insulating substrate which contains a solid phase oxidoreductase and a solid phase ferrocene electrolyte as an electron acceptor, the solid phase reaction layer being formed over at least a portion of the working electrode or the counter electrode,
- wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion, an enzymatic reaction occurs spontaneously between the oxidoreductase substrate, the oxidoreductase and the ferricinium ion of the ferrocene electrolyte, regardless of a potential of the electrode system, and wherein the ferricinium ion is provided in the biosensor together with the oxidoreductase upon dissolution of the reaction layer.
Dependencies are inferred from the claim text.