Capillary system providing multiple analysis of sample from same body of liquid
AssigneeSpectrumedix Corporation
InventorsThomas E. Kane, Li Qingbo
Life of this patent
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Abstract
The present invention provides capillary an electrophoresis system and method for multiple simultaneous analysis of a sample. The system and method of the invention allow the ends of a plurality of capillaries to simultaneously contact a single body of electrophoresis sample contained in a compartment of a container, such as a sample contained in a well of a micro-titer tray.
Claims27
A capillary electrophoresis system for providing simultaneous multiple analysis of a sample, said system comprising:
- at least one assembly of capillaries, said at least one assembly comprising a plurality of capillaries having first and second capillary ends and being held in a spaced relationship relative to one another at a point proximate to said first capillary ends, a cross-section of said first capillary ends defining a minimum bounding polygon having a footprint, the footprint having a major dimension;
- and
- a container comprising at least one compartment having an opening and a bottom, and configured to hold a single body of liquid;
- wherein said at least one assembly is positioned such that the first capillary ends of said plurality of capillaries extend through said opening and into said at least one compartment to thereby simultaneously contact a single body of liquid, when said liquid is contained therein.
A capillary electrophoresis system for providing simultaneous multiple analysis of a sample, said system comprising:
- at least one assembly of capillaries, said at least one assembly comprising a plurality of capillaries having first and second capillary ends and being held in a spaced relationship relative to one another at a point proximate to said first capillary ends, a cross-section of said first capillary ends defining a minimum bounding polygon having a footprint, the footprint having a major dimension;
- and
- a container comprising at least one compartment having an opening and a bottom, and configured to hold a single body of liquid;
- wherein said at last one assembly is positioned such that said first capillary ends of said plurality of capillaries in said assembly extend through said opening and into said at least one compartment with each tip of said first capillary ends terminating at a height no greater than a height h above the bottom of said compartment when a volume of said compartment below said height h is less than about 500 μL.
A method of capillary electrophoresis for providing simultaneous multiple analysis of a sample, said method comprising:
- providing a container having at least one compartment, said compartment having an opening and a bottom, and being configured to contain a sample;
- inserting first ends of a plurality of capillaries having first and second ends through said opening and into a sample contained in said compartment such that said first ends contact said sample;
- applying a voltage potential difference between said first and second ends to thereby cause said sample to migrate from said first ends towards said second ends within each of said plurality of capillaries;
- and
- individually detecting migrated portions of said sample in each of said plurality of capillaries to thereby simultaneously obtain a plurality of separate analyses of said sample.
A method of capillary electrophoresis for providing simultaneous multiple analysis of a sample, said method comprising:
- providing a container having at least one compartment, said compartment having an opening and a bottom, and being configured to contain a sample;
- providing a plurality of capillaries having first and second ends, the capillaries being held in a spaced relationship relative to one another at a point proximate to said first ends;
- inserting the first ends through said opening and into a sample contained in said compartment such that said first ends contact said sample;
- applying a voltage potential difference between said first and second ends to thereby cause said sample to migrate from said first ends towards said second ends within each of said plurality of capillaries;
- and
- detecting migrated portions of said sample in each of said plurality of capillaries.
Dependencies are inferred from the claim text.