US 6,544,734 B1 · Multilayered microfluidic DNA analysis system and method

Abstract

A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

Claims30

Dependent claims

A DNA analysis system comprising:

a sample inlet port;
a cell lysis chamber in fluid communication with said sample inlet port;
a DNA separation chamber in fluid communication with said cell lysis chamber;
a DNA amplification chamber in fluid communication with said DNA separation chamber, said DNA amplification chamber being defined by a substantially monolithic structure, said substantially monolithic structure being formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic particles;
and
a DNA detection system in fluid communication with said DNA amplification chamber.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/460,281Filed 9 Dec 1999 · continuation in part

Priority

9 Oct 1998

Granted

8 Apr 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification71 codes

G01N 30/6069
Body: with compartments or bed substructure
B01J 19/0093
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: microreactors, e.g. miniaturised or microfabricated reactors
B01L 3/502707
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/50273
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…

Family

Application60/103,701Us provisional application · filed 9 Oct 1998

Application09/235,081Parent of this continuation-in-part · filed 21 Jan 1999

Application09/337,086Parent of this continuation-in-part · filed 21 Jun 1999

US 6,572,830 B1Related patent

US 6,592,696 B1Related patent

US 6,544,734 B1This patent

From the US filing history. Foreign family members aren’t listed.

Multilayered microfluidic DNA analysis system and method

Life of this patent

Multilayered microfluidic DNA analysis system and method

Life of this patent

Abstract

Claims30