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US 6,544,732 B1Granted
Encoding and decoding of array sensors utilizing nanocrystals
AssigneeIllumina, Inc.
InventorsMark S. Chee, Steven M. Barnard, Chanfeng Zhao
Life of this patent
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Abstract
Described herein are assays and components for encoding and decoding microspheres. Each assay or component described utilizes at least one nanocrystal.
Claims18
A composition comprising:
- a) a substrate with a surface comprising discrete sites, wherein said discrete sites are wells;
- and
- b) a population of microspheres randomly distributed on said discrete sites, wherein at least one of said microspheres comprises a nanocrystal.
An array composition comprising:
- a) a substrate with a surface comprising discrete sites, wherein said discrete sites are wells;
- and
- b) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises a bioactive agent bound to a target analyte comprising a nanocrystal, wherein said microspheres are randomly distributed on said surface.
An array composition comprising:
- a) a substrate with a surface comprising discrete sites, wherein said discrete sites are wells;
- and
- b) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises:
- i) a bioactive agent;
- and
- ii) an identifier binding ligand bound to a decoder binding ligand comprising a nanocrystal such that the identification of the bioactive agent can be elucidated;
- wherein said microspheres are randomly distributed on said surface.
4 more elements
An array composition comprising:
- a) a substrate with a surface comprising discrete sites, wherein said discrete sites are wells;
- and
- b) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises a bioactive agent bound to a decoder binding ligand comprising a nanocrystal, wherein said microspheres are randomly distributed on said surface.
Dependencies are inferred from the claim text.