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AssigneeMonsanto Technology LLC
InventorsMark J. Rupar, William P. Donovan, Yuping Tan, Annette C. Slaney
Estimating the term…
Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
A purified nucleic acid segment consisting of the nucleic acid complement of SEQ ID NO:1 or a sequence encoding an insecticidally active protein which hybridizes to the sequence of SEQ ID NO:1 under stringent hybridization conditions comprising about 0.02M to about 0.15M NaCl and temperatures of about 50° C. to about 70° C.
A purified nucleic acid segment encoding a B. thuringiensis CryET29 crystal protein of SEQ ID NO:2.
An isolated nucleic acid segment consisting of at least a forty-five basepair contiguous nucleic acid sequence from SEQ ID NO:1.
A biologically pure culture comprising B. thuringiensis NRRL B-21582, or NRRL B-21583.
A method of using a DNA that encodes an isolated CryET29 crystal protein or peptide, comprising the steps of:
A method for detecting a nucleic acid fragment encoding a CryET29 crystal protein, comprising the steps of:
A nucleic acid detection kit comprising, in suitable container means, a CryET29 crystal protein-encoding nucleic acid segment of SEQ ID NO:1 and a detection reagent.
Dependencies are inferred from the claim text.