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US 6,537,752 B1Granted
Temperature control system for polymerase chain reaction
OwnerThomas W. Astleinventor
Life of this patent
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Abstract
In a preferred embodiment, a polymerase chain reaction temperature control system, including: a first tank into which a multiwell sample carrier may be placed; apparatus to introduce first temperature controlled heat transfer medium into the first tank for a first predetermined length of time; and apparatus to subsequently introduce at least a second second temperature controlled heat transfer medium into the first tank for at least a second predetermined length of time.
Claims10
In combination, a polymerase chain reaction temperature control system and a multiwell sample carrier comprising:
- (a) a sample carrier comprising a coil of carrier tape having wells containing therein polymerase chain reaction materials,
- (b) a first tank in which is entirely submerged said sample carrier
- (c) means to introduce first temperature controlled heat transfer medium into said first tank for a first predetermined length of time;
- and
- (d) means to subsequently introduce at least a second temperature controlled heat transfer medium into said first tank for at least a second predetermined length of time.
2 more elements
A method of conducting a polymerase chain reaction comprising:
- (a) providing a first tank in which is entirely submerged a sample carrier comprising a coil of carrier tape having wells containing therein polymerase chain reaction materials;
- (b) introducing a first temperature controlled heat transfer medium into said first tank for a first predetermined length of time;
- and
- (c) subsequently introducing at least a second temperature controlled heat transfer medium into said first tank for at least a second predetermined length of time.
1 more element
Dependencies are inferred from the claim text.