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AssigneeThe Salk Institute for Biological Studies
InventorsMichael M. Neff, Joanne Chory
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The present invention provides cytochrome P450s useful in for producing genetically modified plants characterized as having the phenotypic trait of modulated brassinolide synthesis or signaling, for example, resulting in insect resistance, dwarfism and darker-green foliage compared with wild type plants. Such plants can be modified, for example, using “bas1”, or functional homologues thereof, a polypeptide encoded by bas1 that modulates brassinolide synthesis and/or signaling in plants. The invention also provides methods for modulating ecdysteroid activity in a plant and for assaying brassinosteroid function in a plant. The latter method can be used to create a gain-of-function allelic series of plants characterized by increasing levels of overexpression of a cytochrome P450 to screen for brassinolide activity in a plant species.
A recombinant vector comprising a polynucleotide sequence having at least 95% homology with SEQ ID NO:
An isolated nucleic acid having the nucleotide sequence of SEQ ID NO.:1.
An isolated nucleic acid encoding a polypeptide having the amino acid sequence of SEQ ID NO.:2.
An isolated nucleic acid encoding the amino acid sequence of SEQ ID NO:
An isolated nucleic acid having a cytosine nucleotide at position 784 of SEQ ID NO:
Dependencies are inferred from the claim text.