Back to results
US 6,534,279 B1Granted
Reagent and method for the permeabilization and identification of erythrocytes
AssigneeImmunotech
InventorsAndré Van Agthoven, Christine Fornelli
Life of this patent
Estimating the term…
Abstract
A process for permeabilizing erythrocytes in which the erythrocytes are subjected successively to the action of (a) a fixing agent containing an aliphatic aldehyde and oligosaccharide, (b) a permeabilizing agent containing a detergent and an oligosaccharide, kit for permeabilizing erythrocytes, kit for immuno-marking fetal erythrocytes, and a process for identifying fetal erythrocytes by immuno-marking.
Claims9
A kit for permeabilizing erythrocytes comprising
- (a) a liquid isotonic or hypertonic fixing agent at pH 4 to 7 containing an aliphatic C1-C5 aldehyde which is present in a concentration of from 1.5M to 10M and a disaccharide which is present in a concentration of from 0.025M to 1.32M,
- (b) a permeabilizing agent at pH 3 to 8 containing a detergent selected from the group consisting of Zwitterionic and anionic detergents, and an oligosaccharide;
- and
- (c) two antibodies against fetal antigens which are expressed independently on the fetal erythrocytes.
1 more element
Dependencies are inferred from the claim text.