Gene silencing methods
AssigneePlant Bioscience Limited
InventorsDavid Charles Baulcombe, Olivier Voinnet, Carsten Werner Lederer
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Abstract
Disclosed are methods for silencing a target nucleotide sequence (preferably representing one or more endogenous genes, preferably in a systemic fashion) which is present in a first part of the plant, which method comprises transiently introducing into the cytoplasm of a cell in a second part of the plant, which cell comprises a nucleic acid encoding the target sequence and which is remote from said first part of the plant, a nucleic acid construct.
Claims25
A method for silencing a stably integrated transgene target nucleotide sequence present in a first part of a Nicotiana plant, which method comprises transiently introducing a nucleic acid construct into the cytoplasm of a cell in a second part of the plant, which cell comprises the stably integrated transgene target nucleotide sequence and which is remote from said first part of the plant, wherein said construct:
- (i) encodes a sequence which shares sequence identity with the stably integrated transgene target nucleotide sequence or the complement thereof, and
- (ii) does not encode proteins which are capable of blocking systemic movement of a gene silencing signal, such that a gene silencing signal not comprising the construct initiated in the second part of the plant and propagated to the first part of the plant such as to cause the silencing of said target nucleotide sequence in the first part of the Nicotiana plant.
A method for regulating the expression of a stably integrated transgene target nucleotide sequence present in the first part of a Nicotiana plant, which method comprises transiently introducing a nucleic acid construct into the cytoplasm of a cell in a second part of the plant, which cell comprises stably integrated transgene the target sequence and which is remote from said first part of the plant, wherein said construct:
- (i) encodes a sequence which shares sequence identity with the stably integrated transgene target nucleotide sequence or the complement thereof, and
- (ii) does not encode proteins which are capable of blocking systemic movement of a gene silencing signal, such that a gene silencing signal not comprising the construct is initiated in the second part of the plant and propagated to the first part of the Nicotiana plant thereby regulating expression of the stably integrated transgene target nucleotide sequence.
A method for systemically altering the phenotype of a Nicotiana plant by silencing a stably integrated transgene target nucleic acid in a first part of the plant, which method comprises transiently introducing a nucleic acid construct into the cytoplasm of a cell in a second part of the plant, which cell comprises the stably integrated transgene target nucleotide sequence and which is remote from said first part of the plant, wherein said construct:
- (i) encodes a sequence which shares sequence identity with the stably integrated transgene target nucleotide sequence or the complement thereof, and
- (ii) does not encode proteins which are capable of blocking systemic movement of a gene silencing signal, such that a gene silencing signal not comprising the construct is initiated in the second part of the plant and propagated to the first part of the plant thereby systemically altering the phenotype of the Nicotiana plant.
Dependencies are inferred from the claim text.