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US 6,528,675 B1Granted
Cyanohydrin ethers and esters as high-sensitivity enzyme substrates
AssigneeThe Regents of the University of California
InventorsBruce D. Hammock, Guomin Shan, Rong Zhang
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Abstract
Ethers and esters of cyanohydrins that contain optically detectable moieties are highly effective for detecting, monitoring, and measuring the activity of enzymes that cause the cleavage of certain types of substrates. The cyanohydrins function as proaldehydes and proketones, spontaneously converting to aldehydes and ketones, respectively, which provide a large increase in optical detectability relative to both the starting esters and ethers and the cyanohydrins.
Claims20
A compound having the formula
- in which:
- R1 is an aldehyde or ketone group that is optically detectable by fluorescence emission, UV light absorptivity or colorimetry;
- R2 is a member selected from the group consisting of H, C1-C2 alkyl, benzyl, and substituted benzyl;
- and
- R3 is a member selected from the group consisting of alkyl, substituted alkyl, aralkyl, substituted aralkyl, phosphate, sulfate, carbohydrate residues, substituted carbohydrate residues, and
- in which R4 is a member selected from the group consisting of alkyl, substituted alkyl, aralkyl, substituted aralkyl, oxiranylalkoxy, substituted oxiranylalkoxy, alkylamino, substituted alkylamino, peptidylamino, cycloalkylamino, substituted cycloalkylamino, oxiranylamino, substituted oxiranylamino, alkoxy, and substituted alkoxy;
- R1 being selected such that the optical detectability of
- is measurably greater than the optical detectability of
5 more elements
Dependencies are inferred from the claim text.