Mammalian cell culture process for producing glycoproteins
AssigneeGenentech, Inc.
InventorThomas Ryll
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Abstract
The present invention relates to novel process for the preparation of glycoproteins by mammalian cell culture wherein the sialic acid content of the glycoprotein recovered is optimized by manipulating the cell culture environment. The invention provides for processes in which the sialic acid content of the glycoprotein recovered is increased by addition of copper ion to a cell culture at a concentration effective for stabilization of the sialic acid content.
Claims40
A process for producing a glycoprotein by mammalian cell culture, the process comprising:
- culturing a mammalian host cell in a serum free medium in a production phase which is characterized by adding an effective amount of copper ion to the cell culture, said effective amount of copper ion in a concentration effective to minimize the loss of sialic acid on the glycoprotein.
A process for producing a glycoprotein, by mammalian cell culture comprising the steps of;
- (a) culturing a mammalian host cell which expresses a heterologous glycoprotein in a growth phase under such conditions and for a period of time such that maximum cell growth is achieved;
- (b) culturing the host cell in a production phase in a serum free medium which follows the growth phase wherein the cells are cultured in the presence of copper ion at a concentration of about 50 μM to about 5 mM;
- and
- (c) recovering the glycoprotein so produced.
A process for producing a glycoprotein by mammalian cell culture, the process comprising:
- culturing a mammalian host cell in a serum free medium in a production phase which is characterized by adding an effective amount of copper ion to the cell culture, wherein the copper ion is added to the cell culture at a concentration of about 0.1 mM to about 2.0 mM.
Dependencies are inferred from the claim text.