Hydantoinase variants with improved properties and their use for the production of amino acids
AssigneeCalifornia Institute of Technology
InventorsFrances H. Arnold, Oliver May, Karlheinz Drauz, Andreas Bommarius
Life of this patent
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Abstract
Hydantoinase enzymes which are mutants of a previously isolated hydantoinase having the amino acid SEQ. ID. NO. 2. The mutants include amino acid substitutions at positions 95, 154, 180, 251 and/or 255 of the wild type hydantoinase (SEQ. ID. NO. 2). The mutant hydantoinases, like the parent hydantoinase, are used in the production of optically pure amino acids.
Claims9
A modified hydartoinase consisting of unmodified hydantoinase having SEQ. ID. NO. 2 which has been modified by an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid position numbers 95, 154, 180, 251 and 295.
A modified hydantoinase comprising an unmodified hydantoinase that has an amino acid sequence identity with SEQ. ID. NO. 2 which is about 97.5 percent and wherein said unmodified hydantoinase has been modified by an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid position numbers 95, 154, 251 and 295, and equivalent positions as determined by sequence alignment.
Dependencies are inferred from the claim text.