2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme

An isolated nucleic acid sequence encoding a polypeptide having 2,6-beta-D-fructan hydrolase activity, comprising a nucleic acid sequence encoding:

• an allelic variant of a polypeptide having an amino acid sequence which has at least 90% identity to amino acids 32 to 923 of SEQ ID NO:1.

An isolated nucleic acid sequence encoding a polypeptide having 2,6-beta-D-fructan hydrolase activity, comprising:

• a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 94 to 2769 of SEQ ID NO:2 (ii) the cDNA sequence comprising nucleotides 94 to 2769 of SEQ ID NO:2 or (iii) a complementary strand of (i) or (ii);
• wherein the hybridization conditions comprises hybridization at 42° C. in a solution comprising 5×SSPE, 200 ug/ml sheared and denatured salmon sperm DNA, and 0.3% SDS followed by washing at 65° C. in a solution comprising 2×SSC and 0.2% SDS.

An isolated nucleic acid sequence encoding a polypeptide having 2,6-beta-D-fructan hydrolase activity, comprising the nucleic acid sequence inserted into plasmid pSJ1678 present in E. coli DSM 12406 or the nucleotide sequence inserted into plasmid pSJ1678 present in E. coli DSM 13028 or the nucleotide sequence inserted into plasmid pSJ1678 present in E. coli DSM 13029.