# US 6,521,745 B1 · Nucleic acid molecules encoding inclusion membrane protein C of Chlamydia

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- Number: US 6,521,745 B1
- Document ID: US6521745B1
- Status: Granted
- Kind: B1
- Application number: 09/377,399
- Filed: 1999-08-20
- Granted: 2003-02-18
- Earliest claimed: 1998-08-20
- Assignees: Aventis Pasteur Limited
- Inventors: Andrew D. Murdin; Pamela L. Dunn; Raymond P. Oomen
- Web page: https://patentgrep.com/patent/US6521745B1

## CPC

- C07K 14/295: from Chlamydiales (O)
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- A61K 2039/51: {comprising whole cells, viruses or DNA/RNA}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

An isolated and purified nucleic acid molecule encoding an inclusion membrane protein C of a strain of Chlamydia, is useful for nucleic acid immunization of a host, including a human host, against disease caused by infection by a strain of Chlamydia, particularly C. pneumoniae.

## Claims

### Claim 1

An isolated and purified nucleic acid molecule encoding an inclusion membrane protein C of a strain of Chlamydia having a polynucleotide sequence selected from the group consisting of:
(a) a polynucleotide sequence having SEQ ID Nos: 1 or 2 or the complementary polynucleotide sequence thereto, and
(b) a polynucleotide sequence encoding an amino acid sequence having SEQ ID No: 3.

### Claim 2 (depends on 1)

The nucleic acid molecule of claim 1 which is retrieved from the strain of Chlamydia by PCR amplification of genomic bacterial DNA using synthetic oligonucleotide primers having a nucleotide sequence complementary to 5′ and 3′ ends of the encoding domain.

### Claim 3 (depends on 2)

The nucleic acid molecule of claim 2 wherein each said primers consist of about 10 to 40 nucleotides.

### Claim 4 (depends on 2)

The nucleic acid molecule of claim 2 wherein each said primers consist of about 15 to 25 nucleotides.

### Claim 5 (depends on 3)

The nucleic acid molecule of claim 3 wherein each said primers contains at least about 40% of the nucleotides which are C and G nucleotides to ensure efficient hybridization.

### Claim 6 (depends on 4)

The nucleic acid molecule of claim 4 wherein each said primers contains at least about 50% of the nucleotides which are C and G nucleotides to ensure efficient hybridization.