Gene construct and its use
AssigneeGSF-Forschungszentrum fur Umwelt und Gesundheit GmbH
InventorsAxel Polack, Konstanze Hörtnagel, Jürgen Wolf, Susanne Mücke
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Abstract
Compositions of matter and methods for expressing a polypeptide are provided. In one aspect, a gene construct is provided that comprises an enhancer comprising particular elements or combinations of elements from the immunoglobulin κ locus, the immunoglobulin heavy chain μ locus, and the immunoglobulin λ locus, and a promoter and polyadenylation site linked to a nucleotide sequence encoding a polypeptide from a select group. Cells comprising the gene construct, processes for producing such cells, and processes for producing the polypeptide encoded by the gene construct from such cells are also provided.
Claims36
A gene construct for the expression of a polypeptide, comprising:
- (a) an enhancer comprising an element or combination of elements, wherein said element or combination of elements is selected from the group consisting of:
- (i) a combination comprising the following elements obtained from the immunoglobulin κ locus:
- the κ intron enhancer (κEi), the κ 3′ enhancer (κE3′), and the immunoglobulin κ matrix attachment region (κMAR);
- (ii) a combination comprising the following elements obtained from the immunoglobulin heavy chain μ locus:
- the μ0 intron enhancer (μEi) and the μ 3′ enhancer obtained from a region located 3′ of Cα (μE3′);
- (iii) a combination of
- one or more elements selected from the group consisting of κEi and κE3+ and
- one or more elements selected from the group consisting of μEi and μE3′;
- (iv) the enhancer element of the immunoglobulin λ locus (λE);
- (v) a combination of
- λE and
- one or more elements selected from the group consisting of κEi and κE3′;
- and
- (vi) a combination of
- λE and
- one or more elements selected from the group consisting of μEi and μE3′;
- (b) a promoter operably linked to said enhancer;
- (c) a nucleotide sequence operably liked to said promoter, said nucleotide sequence encoding a polypeptide selected from the group consisting of:
- a cytokine, a viral antigen, a cellular adhesion protein, a tumor antigen with the proviso that the c-myc-antigen is excluded, a co-stimulatory signalling molecule, an HLA antigen, and β-galactosidase;
- and
- (d) a polyadenylation site (PAA) operably linked to said nucleotide sequence.
Dependencies are inferred from the claim text.