Nucleic acids encoding polypeptides having haloperoxidase activity
AssigneesNovozymes A/S, Maxygen, Inc.
InventorsSteffen Danielsen, Palle Schneider
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Abstract
The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.
Claims17
An isolated nucleic acid sequence comprising a nucleic acid sequence which encodes a polypeptide having haloperoxidase activity, wherein the polypeptide is selected from the group consisting of:
- a) a polypeptide having an amino acid sequence which has at least 95% homology with the amino acid sequence of SEQ ID NO:2;
- b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with (i) the nucleotide sequence of SEQ ID NO:1, (ii) a subsequence of (i) of at least 100 contiguous nucleotides, or (iii) a complementary strand of (i) or (ii);
- c) a fragment of (a), or (b) that has haloperoxidase activity;
- and
- d) a polypeptide of (a) or (b) having more than 50% residual activity after 15 minutes incubation at 70° C. and pH 7.
An isolated nucleic acid sequence comprising a nucleic acid sequence having at least one mutation in the polypeptide coding sequence of SEQ ID NO:1, in which the mutant nucleic acid sequence encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:2.
A method for producing a mutant nucleic acid sequence, comprising (a) introducing at least one mutation into the polypeptide coding sequence of SEQ ID NO:1, wherein the mutant nucleic acid sequence encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:2;
- and (b) recovering the mutant nucleic acid sequence.
A method for producing a polypeptide comprising (a) cultivating a host cell under conditions conducive for production of the polypeptide, wherein the host cell comprises a mutant nucleic acid sequence having at least one mutation in the polypeptide coding sequence of SEQ ID NO:1, wherein the mutant nucleic acid sequence encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, and (b) recovering the polypeptide.
A method for shuffling of DNA comprising using the nucleotide sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.