Purified telomerase
AssigneeGeron Corporation
InventorsScott L. Weinrich, Edward M. Atkinson, III, Serge P. Lichtsteiner, Alain P. Vasserot, Ronald A. Pruzan
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Abstract
This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.
Claims20
A preparation of mammalian telomerase protein that is at least ˜3,000-fold more pure (in terms of telomerase activity per weight of protein) than a crude extract of 293 cells, wherein a complex of the telomerase protein with telomerase RNA component has a molecular weight of 200-2,000 kDa.
Purified human telomerase protein having at least 2,000-fold increased purity compared with crude extract of cells from adenovirus-transformed kidney cell line (293 cells), which when associated with telomerase RNA component has DNA polymerase activity and a molecular weight of 200-2,000 kDa.
An extract of a cell expressing mammalian telomerase protein, wherein the extract has measurable telomerase activity in 0.2 μg of protein when quantified in a telomere primer elongation assay in which 32P-labeled primer extensions are separated on a gel and detected using a phosphoimager screen.
Dependencies are inferred from the claim text.