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US 6,514,739 B1Granted
Lysophospholipase
OwnerHiroaki Udagawa, Torben Peter Frandsen +3inventors
InventorsHiroaki Udagawa, Torben Peter Frandsen, Tom Anton Busk Nielsen, Markus Sakari Kauppinen, Søren Christensen
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Abstract
The inventors have isolated lysophospholipases from Aspergillus (A. niger and A. oryzae) having molecular masses of about 68 kDa and amino acid sequences of 600-604 amino acid residues. The novel lysophospholipases have only a limited homology to known amino acid sequences. The inventors also isolated genes encoding the novel enzymes and cloned them into E. coli strains.
Claims13
An isolated lysophospholipase comprising:
- a) a polypeptide encoded by a lysophospholipase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli deposit number DSM 13004;
- b) a polypeptide having an amino acid sequence of amino acids 1-600 in SEQ ID NO:
- 4;
- c) an analogue of the polypeptide defined in (a) or (b) which has at least 95% sequence homology with said polypeptide;
- or
- d) a polypeptide which is encoded by a nucleic acid sequence which hybridizes with a complementary strand of the nucleic acid sequence shown as nucleotides 115-1914 of SEQ ID NO:
- 3 under hybridization conditions comprising prehybridizing in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approx. 45° C., followed by washing in 2×SSC, 0.5% SDS for 30 minutes at a temperature of at least 65° C.
4 more elements
Dependencies are inferred from the claim text.