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US 6,511,853 B1Granted
Optimized high-throughput analytical system
AssigneeCaliper Technologies Corp.
InventorsAnne R. Kopf-Sill, Andrea W. Chow
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Abstract
Throughput rates for microfluidic serial analysis systems are optimized by maximizing the proximity and speed with which multiple different samples may be serially introduced into a microfluidic channel network. Devices are included that include optimized parameters based upon desired throughput rates for a given set of reagents, reaction times and the like.
Claims15
A method for performing a serial analysis in a microfluidic channel network, comprising:
- selecting a cycle length of a test plug containing a test compound plug and a spacer plug;
- identifying a diffusion coefficient of the test compound in the test compound plug;
- selecting a total reaction time for the test compound;
- determining one or more of a maximum channel cross-sectional dimension and a channel length for carrying out the analysis, based upon the cycle length, diffusion coefficient and total reaction time, whereby the test compound in the test compound plug disperses a distance that is less than 200% of the test compound plug length during the total reaction time;
- providing at least a first microfluidic channel that comprises one or more of the maximum channel cross-sectional dimension and channel length;
- introducing at least a first test plug having the cycle length into the at least first microfluidic channel;
- and
- analyzing the test compound in the first microfluidic channel.
5 more elements
Dependencies are inferred from the claim text.