Method for localizing allantoic fluid of avian eggs
AssigneeEmbrex, Inc.
InventorsAnn K. Gore, Thomas E. Bryan
Life of this patent
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Abstract
Methods of pooling or localizing the allantoic fluid present in avian shell eggs, so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle or probe traveling in a downward direction.
Claims29
A method for directing a probe into the allantois of an avian egg which comprises:
- selecting an avian egg in the second or third quarter of incubation;
- orienting the long axis of the egg to a predetermined angle from about 10 degrees to 180 degrees from vertical wherein the large end of the egg in an upwardly vertical position defines zero degrees vertical, thereby causing the allantois of the egg to pool and enlarge the allantoic sac under the upper portion of the egg shell;
- and
- inserting a probe through the shell of the egg and into the enlarged allantoic sac.
A method of removing a sample of allantoic fluid from a chicken egg which comprises:
- selecting a chicken egg in the 12th to 16th day of incubation;
- orienting the long axis of the egg to a predetermined angle from about 10 degrees to 180 degrees from vertical wherein the large end of the egg in an upwardly vertical position defines zero degrees vertical, thereby causing the allantois of the egg to pool and enlarge the allantoic sac under the upper portion of the egg shell;
- inserting a sampling device downwardly through the shell of the egg and directly into the enlarged allantoic sac;
- and
- removing a sample of allantoic fluid from the egg with the sampling device.
A method for directing a probe into the allantois of a plurality of chicken eggs which comprises:
- selecting a plurality of chicken eggs in about the 5th to 18th day of incubation;
- causing said selected eggs to be oriented on an egg tray to a predetermined angle from about 10 degrees to 180 degrees from vertical, wherein zero degrees vertical is defined by the large end of the egg in a vertically upward position, whereby the allantois of each egg is caused to pool and enlarge the allantoic sac in a predetermined area of the egg under the upper portion of the egg shell;
- inserting a probe downwardly into each egg through the shell of the egg and into the enlarged allantoic sac in the predetermined area.
Dependencies are inferred from the claim text.