Oil bodies and associated proteins as affinity matrices
AssigneeSemBioSys Genetics Inc.
InventorsMaurice Moloney, Joseph Boothe, Gijs Van Rooijen
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Abstract
A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions
Claims28
A method for the separation of a target molecule from a sample comprising:
- 1) contacting (i) oil bodies with (ii) a sample containing the target molecule to allow the target molecule to associate with the oil bodies;
- and
- 2) separating the oil bodies associated with the target molecule thereby separating the target molecule from the sample.
Dependencies are inferred from the claim text.