Process for preparing docosahexaenoic acid and docosapentaenoic acid with ulkenia
AssigneesSuntory Limited, Nagase & Co., Ltd., Nagase Chemtex Corporation
InventorsSatohiro Tanaka, Toshiaki Yaguchi, Sakayu Shimizu, Tsutomu Sogo, Shigeaki Fujikawa
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Abstract
A process for preparing lipids which contain docosahexaenoic acid (DHA) and/or docosapentaenoic acid (DPA) is disclosed. The process includes the steps of cultivating in a medium a microorganism which belongs to the genus Ulkenia having the ability to produce DHA and/or DPA and recovering the lipids from the culture. The process may further include the step of separating DHA and/or DPA from the lipids.
Claims4
A process for preparing lipids containing docosahexaenoic acid and/or docosapentaenoic acid, the process comprising:
- providing a culture, the culture comprising a culture medium and the microorganism Ulkenia sp. SAM 2179, FERM BP-5061;
- cultivating the microorganism in the culture medium under conditions suitable for the production of lipids containing docosahexaenoic acid and/or docosapentaenoic acid;
- and
- recovering said lipids from the culture.
A process for preparing docosahexaenoic acid, the process comprising:
- cultivating in a nutrient medium the microorganism Ulkenia sp. SAM 2179, FERM BP-5061;
- recovering lipids from the medium;
- hydrolyzing said lipids to prepare docosahexaenoic acid;
- and
- recovering said docosahexaenoic acid.
A process for preparing docosapentaenoic acid, the process comprising:
- cultivating in a nutrient medium the microorganism Ulkenia sp. SAM 2179, FERM BP-5601;
- recovering lipids from the medium;
- hydrolyzing said lipids to prepare docosapentaenoic acid;
- and
- recovering said docosapentaenoic acid.
A process for preparing structured lipids containing docosahexaenoic acid or docosapentaenoic acid, the process comprising:
- cultivating in a nutrient medium the microorganism Ulkenia sp. SAM 2179, FERM BP-5061;
- culturing the microorganism for a sufficient time to produce lipids containing triacylglycerol having long chain fatty acids at positions 1 to 3 of its glycerol backbone, wherein the long chain fatty acid of at least position 2 comprises docosahexaenoic acid or docosapentaenoic acid;
- recovering said lipids from the medium;
- treating said lipids with fungal lipase and a source of medium chain fatty acids to convert the long chain fatty acids at positions 1 and 3 of said glycerol backbone of the triacylglycerol into medium chain fatty acids;
- and
- recovering said structured lipids.
Dependencies are inferred from the claim text.