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US 6,509,171 B1Granted
Aspartic proteinase deficient filamentous fungi
AssigneeGenencor International, Inc.
InventorsRandy M. Berka, Kirk J. Hayenga, Virgil B. Lawlis, Michael Ward
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Abstract
This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.
Claims15
A method of producing a heterologous polypeptide in a mutant filamentous fungus which comprises:
- culturing a mutant filamentous fungus which is capable of expressing the heterologous polypeptide and which contains a non-revertable site-selected deletion of part or all of nucleic acid coding for an aspartic proteinase produced therefrom that results in the mutant filamentous fungus being incapable of excreting enzymatically active aspartic proteinase;
- and recovering the heterologous polypeptide,
- wherein said nucleic acid coding for said aspartic proteinase hybridizes to an oligonucleotide probe coding for 6-20 amino acids of the mature aspartic proteinase.
Dependencies are inferred from the claim text.