System for polymerizing collagen and collagen composites in situ for a tissue compatible wound sealant, delivery vehicle, binding agent and/or chemically modifiable matrix
AssigneeBoard of Regents, Texas A&M University System
InventorsDouglas R. Miller, Ian R. Tizard, Jimmy T. Keeton, Jerry F. Prochaska
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Abstract
This invention relates to materials and methods for crosslinking, in situ, proteins, including collagen, with peroxidase, including horseradish peroxidase, to from biocompatible semi-solid gels useful in a number of biological and food product applications.
Claims30
A method of sealing a wound comprising the steps of:
- applying to a wound a mixture of collagen and a crosslinking agent, wherein the collagen is selected from the group consisting of Type I collagen, Type II collagen, Type III collagen and Type IV collagen and the crosslinking agent is peroxidase and hydrogen peroxide (H2O2);
- crosslinking said collagen in said wound, whereby said crosslinking forms a semi-solid gel that seals said wound.
A method of forming a semi-solid crosslinked polymer on the surface of meat or poultry tissues for use as a food binding/restructuring agent comprising the steps of crosslinking a protein with a peroxidase in the presence of peroxide, wherein the protein is selected from the group consisting of Type I collagen, Type II collagen, Type III collagen, and Type IV collagen, fibrinogen, and recombinant fibronectin engineered protein polymer, and the peroxidase is selected from the group consisting of horseradish peroxidase, soybean peroxidase, and microbial peroxidase from Arthromyces ramosus.
A method for growing dermal fibroblasts in vitro comprising the steps of growing the fibroblasts in a peroxide crosslinked collagen polymer comprising a three dimensional matrix for the growth of said dermal fibroblasts in vitro.
Dependencies are inferred from the claim text.