Chromatography detection system and method
OwnerEdward Rapkininventor
Life of this patent
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Abstract
A chromatography detection system can handle the flow from a chromatography column. The chromatography detection system has an injection valve and a detector for detecting emissions from a flowing sample. The injection valve has at least one loop and is adapted to connect to the chromatography column. The valve can (a) load the at least one loop independently of the chromatography column in a first mode, and (b) serially connect the least one loop between the chromatography column and the detector in a second mode. Thus, flow from the at least one loop to the detector is driven by eluate from the chromatography column.
Claims15
A chromatography detection method employing an injection valve with at least one loop and a flow-through emissions detector, for handling the flow from a chromatography column, comprising the steps of:
- loading said at least one loop with an independent sample having a known activity and including a standard quantity of an isotope diluted with mobile phase as employed in said chromatography column, said independent sample being derived from an independent source independently of said chromatography column in a first mode without connecting said at least one loop to either said flow-through detector or said chromatography column;
- serially connecting said at least one loop between said chromatography column and said flow-through emissions detector in a second mode for unloading said independent sample;
- causing said independent sample to flow by driving a flow from said at least one loop to said detector with eluate from said chromatography column;
- detecting emissions in said second mode as said independent sample flows through said flow-through emissions detector;
- determining efficiency of said emissions detector by calculating the efficiency of detected emissions with respect to the known activity of said independent sample based on the flow of said independent sample through said emissions detector;
- detecting emissions when said independent sample is absent from said emissions detector and eluate from said chromatography column is present in said emissions detector;
- and
- correcting emissions readings for flow from said chromatography column using the efficiency determined from the flow of said independent sample through said emissions detector.
A chromatography detection method employing an injection valve with one or more loops and a flow-through emissions detector, for handling the flow from a chromatography column, wherein said chromatography column receives samples having a distinct pair of isotopes, comprising the steps of:
- loading said one or more loops with a first independent sample derived from an independent source derived independently of said chromatography column, said first independent sample including a standard quantity of one of said distinct pair of isotopes having a known activity and being diluted with mobile phase as employed in said chromatography column;
- loading into said one or more loops, at a different time, a second independent sample having a known activity and including a standard quantity of a different one of said distinct pair of isotopes diluted with mobile phase as employed in said chromatography column, said first and said second independent sample being derived independently of said chromatography column without connecting said one or more loops to said chromatography column;
- serially connecting said one or more loops repeatedly between said chromatography column and said flow-through emissions detector in order to unload said first and said second independent sample at different times;
- measuring the energy distribution indicated by said emissions detector as different ones of said isotopes from said first and said second independent sample flow through said detector, noting the fractions above and below a predetermined energy gap;
- and
- correcting measurements by said emissions detector of eluate from said chromatography column based on the extent of overlapping of energy distributions for said pair of isotopes relative to said predetermined energy in order to correct for spillover effects in eluate from said chromatography column.
A chromatography detection method employing one or more loops and a flow-through emissions detector, for handling the flow from a chromatography column, wherein said chromatography column receives at least two, different eluants in time varying proportions, comprising the steps of:
- loading into said one or more loops a first independent sample derived from an independent source independently of said chromatography column and having a predetermined isotope with a first one of said two different eluants;
- loading into said one or more loops a second independent sample having said predetermined isotope with a second one of said two different eluants, said first and said second independent sample being derived independently of said chromatography column without connecting said one or more loops to said chromatography column;
- serially connecting said one or more loops between said chromatography column and said flow-through emissions detector in order to unload said first and said second independent sample at different times;
- measuring the response of said emissions detector to said first and said second independent sample as said isotope flows through said detector at separate times to produce separate values corresponding to elution with different compositions of said eluants;
- and
- correcting measurements by said emissions detector of eluate from said chromatography column that was mixed with said different eluant compositions by using different corresponding ones of said separate values, so that the effect of gradient elution can be evaluated and used to correct for inefficiencies such as quenching.
Dependencies are inferred from the claim text.